Structural analysis of water-soluble fractions obtained fromAspergillus fumigatus mycelium |
| |
| Authors: | Veronica M Hearn Beatrice L Griffiths Philip A J Gorin |
| |
| Institution: | (1) Mycological Reference Laboratory, Central Public Health Laboratory, 61 Colindale Avenue, NW9 5HT London, England;(2) MRC Human Biochemical Genetics Unit, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE London, England;(3) Departamento de Bioquimica, CP19046, Universidade Federal do Parana, 81.504 Curitiba-Pr., Brazil |
| |
| Abstract: | Fractions were prepared from the water-soluble components ofAspergillus fumigatus mycelium either by lectin-affinity chromatography or salt precipitation. While they varied considerably in their amino-acid composition, each contained a preponderance of aspartic and glutamic acids.13C-NMR spectroscopy of these fractions, compared with that of polysaccharide obtained by alkaline extraction, indicated the presence of glycoproteins, the polysaccharide components of which contained  -d-Galf units that are part of structures chemically different from those obtained by alkali treatment. In two of the three fractions examined, gas-liquid chromatography-mass spectrometry showed marked differences in the contents of non-reducing end-units of  -d-Manp and -d-Galf. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the preparations revealed an array of components, which stained to differing extents with silver stain and with Coomassie Blue and many of which were bound by lectins with specificity for different sugars. |
| |
| Keywords: | GLC-MS 13
C-NMR SDS-PAGE lectin binding |
| 本文献已被 SpringerLink 等数据库收录！ |
|
