Effects of defined medium,fetal bovine serum,and human serum on growth and chemosensitivities of human breast cancer cells in primary culture: Inference for in vitro assays |
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Authors: | Joanne T Emerman Eveline E Fiedler Anthony W Tolcher Patricia M Rebbeck |
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Institution: | (1) Department of Anatomy, University of British Columbia, 2177 Wesbrook Mall, V6T 1W5 Vancouver, British Columbia, Canada;(2) Department of Surgery, University of British Columbia, 2177 Wesbrook Mall, V6T 1W5 Vancouver, British Columbia, Canada |
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Abstract: | Summary We compared the effects of defined medium, fetal bovine serum (FBS) and human serum (HuS) on the growth and responses to chemotherapeutic
agents of human breast cancer cells in primary culture. Normal and tumor tissues were dissociated to small aggregates and
single cells and seeded onto collagen-gel-coated wells in defined medium or medium supplemented with 5% FBS or 5% HuS. In
all cases examined, defined medium and medium containing HuS were superior to medium containing FBS in supporting growth of
both normal and tumor cell cultures. However, cultures in defined medium showed an initial cell loss. Cells from the same
tumor cultured in different media varied in their responses to chemotherapeutic agents. In light of these results, medium
supplemented with HuS, which promoted attachment of these cells in culture and stimulated their growth, should be the most
appropriate nutrient environment for determining the effects of therapeutic agents on cells as it most closely resembles the
in vivo situation. Because there were also variations in growth rates and chemosensitivities of tumor cells cultured in different
human serum samples, we suggest that optimal conditions in which to culture these cells include the serum of the patient whose
tumor is removed. This serum may provide host factors that influence cell growth and interact with exogenous factors.
This work was supported by a grant from the National Cancer Institute of Canada and funds contributed by Mr. B. T. Wharton
in memory of his wife, Nadia. J. T. Emerman is a research scholar of the National Cancer Institute of Canada. |
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Keywords: | human breast cancer defined medium fetal bovine serum human serum chemotherapeutic agents |
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