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Exon Mapping by Fiber-FISH or LR-PCR |
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| Authors: | Ralph J Florijn Frans M van de Ruke Hans Vrolijk Lau A J Blonden Marten H Hofker Johan T den Dunnen Hans J Tanke Gert-Jan B van Ommen Anton K Raap |
| Institution: | aDepartment of Cytochemistry and Cytometry, State University Leiden, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands;bDepartment of Human Genetics, State University Leiden, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands |
| Abstract: | In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of ≥200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations. |
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