The bovine major histocompatibility complex (BoLA): Close linkage of the genes controlling serologically defined antigens and mixed lymphocyte reactivity |
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| Authors: | Dr. William R. Usinger Martin Curie-Cohen Kathy Benforado Deborah Pringnitz Robert Rowe Gary A. Splitter William H. Stone |
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| Affiliation: | (1) Department of Veterinary Science, University of Wisconsin-Madison, 1655 Linden Drive, 53706 Madison, Wisconsin;(2) Department of Genetics, University of Wisconsin-Madison, 53706 Madison, Wisconsin |
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| Abstract: | Detection of linkage between genetic loci in cattle has been hampered by the lack of large full-sib families. A unique source of full-sib families is now available from embryo transplantation. Lymphocytes from six full-sib families, ranging in size from three to seven siblings, were tested for serologically defined BoLA antigens (BoLA-A). In addition, mixed lymphocyte reactivity (MLR) was tested between all paired combinations of cells within each family to distinguish BoLA-D specificities. Serologically identical siblings within each family were reciprocally nonreactive in MLR, and vice versa; thus, no recombinants were detected between the BoLA-A and the BoLA-D loci. Classical genetic linkage analysis revealed that these loci are significantly closer than 11.9 centimorgans. |
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