混合培养中巨大芽孢杆菌对氧化葡萄糖酸杆菌的作用

为查明维生素Ｃ二步发酵混合培养中巨大芽孢杆菌与氧化葡萄糖酸杆菌间的关系，通过生长曲线测定、静息细胞实验及摇瓶发酵实验研究了巨大芽孢杆菌对氧化葡萄糖酸杆菌生长和产生２－酮基－Ｌ－古龙酸作用的影响；采用超滤分离、凝胶层析及聚丙烯酰胺凝胶电泳技术对巨大芽孢杆菌胞外液中具有促进氧化葡萄糖酸杆菌产酸作用的活性物质进行了分离和纯化。结果表明，大菌胞内液和胞外液均可促进小菌生长，大菌胞外液中具有该作用的组分分子

Effect of Bacillus megaterium on Gluconobacter oxydans in mixed culture

To reveal the relationship between Bacillus megaterium and Gluconobacter oxydans in the mixed culture of vitamin C two-step fermentation, the effect of B. megaterium on the growth of G. oxydans and its synthesizing ability of 2-keto-L-gulonic acid(2KGA) was studied. The bioactive metabolites which could enhance the synthesis of 2KGA were isolated and purified by ultrafiltration, gel chromatography and SDS-polyacrylamide gel electrophoresis. Both the culture supernatant and the cytosol of B. megaterium could promote the proliferation of G. oxydans, and the active component in the culture supernatant was above 100 KDa. The culture suernatant could enhance the conversion of L-sorbose to 2KGA, while the cytosol could not. The active components in B. megaterium culture supernatant had molecular weight of 30-50 KDa and above 100 KDa, and the former was a kind of protein with an apparent molecular weight of about 35 KDa, which consisted of one sort of subunit and contained Fe and Zn elements.