Podophyllotoxin production via cell and adventitious root cultures of <Emphasis Type="Italic">Podophyllum peltatum</Emphasis> |
| |
Authors: | V R Anbazhagan C H Ahn E Harada Y S Kim Y E Choi |
| |
Institution: | (1) Forest Resources Development, Division of Forest Resources, College of Forest Sciences, Kangwon National University, Chunchon, 200-701, Kangwon-do, Korea;(2) Laboratory for Plant Biotechnology, Pharmax Co., Ansung, 456-756, Korea;(3) Present address: Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma 630-0192, Nara 630-0192, Japan |
| |
Abstract: | Podophyllum peltatum is an important medicinal plant that produces podophyllotoxin (PTOX) with anti-cancer properties. We established the embryogenic
cell and adventitious root culture systems in P. peltatum and analyzed PTOX production. For the growth of embryogenic cell clumps in shake flask culture, the most efficient concentration
of 2,4-dichloroacetic acid (2,4-D) was 6.78 μM, and the growth of embryogenic cell clumps was 15.9-fold increased in Murashige
and Skoog MS liquid medium with 6.78 μM 2,4-D after 3 wk of culture. To induce adventitious roots, half-strength MS medium
showed the best results for adventitious root induction compared to full strength MS medium and MS medium lacking NH4NO3. Optimal indole-3-butyric acid concentration for adventitious root formation was 14.78 μM. In liquid medium, the frequency
of adventitious root formation from root segments was 87.7% and the number of laterally formed adventitious roots was 22.3
per segment. PTOX production in embryogenic cells and adventitious roots was confirmed by liquid chromatography and electrospray
ionization–tandem mass spectrometry analysis. High-performance liquid chromatography analysis revealed that adventitious roots
contained higher PTOX than embryogenic cell clumps. Elicitor treatment (20 μM methyl jasmonate) strongly enhanced the production
of PTOX in both embryogenic cell clumps and adventitious roots. This observation suggests that both embryogenic cell and adventitious
root culture can be adopted to produce PTOX. |
| |
Keywords: | Adventitious root induction Podophyllotoxin Cell suspension culture Elicitor |
本文献已被 SpringerLink 等数据库收录! |
|