白背飞虱、灰飞虱mariner转座子的初步研究

根据毛里塔尼亚果蝇Drosophila mauritania的mariner转座子序列设计简并引物，以白背飞虱Sogatella furcifera和灰飞虱Laodelphax striatellus基因组DNA为模板，通过PCR反应扩增出了约500 bp的条带，经回收、克隆、测序，获得其核苷酸序列，与毛里塔尼亚果蝇mariner序列相比，核苷酸同源性均为71.3%，氨基酸同源性分别为69.5%和72%，证明白背飞虱和灰飞虱体内确实存在mariner转座子。

The mariner transposable element of Sogatella furcifera and Laodelphax striatellus

About 500 bp DNA fragment is amplified by PCR with the degenerated primers designed according to the mariner sequence of Drosophila mauritiana, the genomic DNA of either Sogatella furcifera or Laodelphax striatellus as the template. Sequence analysis shows that the fragment similarity of both the DNA to that of D. mauritiana is 71 3%, and the similarity of the amino acid is 69 5% and 72% respectively. This indicates that the mariner element may exist in S. furcifera and L. striatellus.