Extent of ubiquinone reduction by succinate in electron transport particles from beef heart |
| |
| Authors: | B T Storey |
| |
| Affiliation: | 1. American University of Beirut Medical Center, Pathology and Laboratory Medicine Department, Cairo Street, Hamra, Beirut, Lebanon;2. Université Paris Cité, Inserm, Maladies neurodéveloppementales et neurovasculaires, F-75019 Paris, France;3. Faculty of Medicine and Health Technology, FI-33014, Tampere University, Finland;4. Institute of Biotechnology, University of Helsinki, Helsinki, Finland |
| |
| Abstract: | Addition of millimolar succinate to a buffered suspension of beef heart electron transport particles (ETP) treated with cyanide gives a greater decrease in optical absorption at 275 vs. 290 mμ, corresponding to ubiquinone (Q) reduction, than does addition of cyanide to succinate-treated particles prior to anaerobiosis. In the latter case, addition of millimolar succinate to the aerobic suspension rapidly reduces about one fourth of the Q, which level is maintained during steady state oxidation of succinate to fumarate. If the absorption decrease corresponding to this reduction, which is obscured by an increase due to fumarate, is accounted for, then the same total absorption decrease is observed for both cases of cyanide-induced and natural anaerobiosis; it corresponds to complete Q reduction. A pigment with a difference spectrum peak (reduced minus oxidized) at 313 mμ is also observed in these ETP; it interferes with Q determination if reference wavelengths longer than 290 mμ are used. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
