Increased calcium in carrots by expression of an Arabidopsis H+/Ca2+ transporter |
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| Authors: | Park Sunghun Kim Chang-Kil Pike Leonard M Smith Roberta H Hirschi Kendal D |
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| Institution: | (1) Present address: Vegetable and Fruit Improvement Center, Texas A&M University, College Station, TX, 77845;(2) Dept. of Horticulture, Sang-Ju National University, 742-711 Kyungpook, Korea;(3) Baylor College of Medicine, Depts of Pediatrics, and Human and Molecular Genetics, USDA/ARS Children's Nutrition Research Center, 1100 Bates St., Houston, TX, 77030 |
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| Abstract: | We demonstrate that carrots expressing the Arabidopsis H+/Ca2+ transporter CAX1 (Cation Exchanger 1) contained up to 50% more calcium (Ca) than plants transformed with control vectors. The CAX1-expressing carrots were fertile, and robust plant growth was seen in the majority of the transgenic plants. CAX1-expressing carrots were crossed to a commercial carrot variety to confirm that the increased Ca accumulation was mediated by CAX1-expression, and the increased Ca content was clearly correlated with the transgene. This study suggests that modulation of ion transporters could be an important means of increasing the Ca content of agriculturally important crops. To our knowledge, this study represents the first attempts to use biotechnology to increase the Ca content of an agriculturally important crop. |
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| Keywords: | Arabidopsis Calcium Carrot H+/Ca2+ transporter Nutrition |
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