Immunodiffusion test for diagnosing human pythiosis |
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| Authors: | P Imwidthaya S Srimuang |
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| Institution: | (1) Department of Microbiology, Siriraj Hospital, Mahidol University, 10700 Bangkok, Thailand;(2) Division of Infectious Disease and Host Defence Unit, Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, 10400 Bangkok, Thailand |
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| Abstract: | An immunodiffusion test was developed for diagnosing subcutaneous and systemic pythiosis in humans. When culture filtrate antigen (CFA) from P. insidiosum was reacted against patient and rabbit antisera, 1–5 precipitin bands occured both in patient and rabbit antisera, and a line of identity also occured between patient and rabbit sera. When control P. insidiosum CFA was reacted with 30 apparently normal persons, 20 Thalassemia patients, 2 candidosis and 5 aspergillosis patients, no precipitin bands were found. P. insidiosum CFA also tested with rabbit antibodies to B. dermatitidis, C. immitis, H. capsulatum, P. brasiliensis, C. albicans, M. furfur and A. fumigatus revealed no cross reactions. This test is practical, sensitive and specific. |
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| Keywords: | human pythiosis immunodiffusion |
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