Gene Disruption by Homologous Recombination in the Xylella fastidiosa Citrus Variegated Chlorosis Strain |
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Authors: | Patrice Gaurivaud Leonardo C A Souza Andrea C D Virgílio Anelise G Mariano Renê R Palma and Patrícia B Monteiro |
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Institution: | Patrice Gaurivaud, Leonardo C. A. Souza, Andrea C. D. Virgílio, Anelise G. Mariano, Renê R. Palma, and Patrícia B. Monteiro |
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Abstract: | Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for β-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to β-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant. |
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