Culture of cardiac muscle cells in serum-free media |
| |
Authors: | W C Claycomb |
| |
Institution: | Department of Biochemistry, Louisiana State University School of Medicine, New Orleans, LA 70112, USA |
| |
Abstract: | Cardiac muscle cells from neonatal rats have been cultured in completely defined serum-free media. The most successful system consists of precoating culture flasks with fibronectin at a concentration of 5 μg/cm2 of surface area and adding fetuin and either dibutyryl cyclic AMP (db-cAMP), cholera toxin, epidermal growth factor or insulin plus dexamethasone to the medium. In order to define a serum- and a hormone or growth factor-free medium, cardiac muscle cells were grown in the presence of fibronectin, fetuin and db-cAMP for 4 days, after which time db-cAMP was omitted from the medium. Under these conditions the cells continue to maintain their differentiated morphology for at least 4 days thereafter. These morphological studies demonstrate that dissociated neonatal cardiac muscle cells are able to grow and differentiate in a chemically defined medium in the absence of animal serum. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|