Sequence Analysis of the Internal Transcribed Spacer 2 (ITS2) from Yeast Species Within the Genus Candida |
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Authors: | Timothy J Lott Brian M Burns Rosely Zancope-Oliveira Cheryl M Elie Errol Reiss |
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Institution: | (1) Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention and Public Health Service, U.S. Department of Health and Human Services, Bldg. 5, B/12 mailstop G-11, 1600 Clifton Road NE, Atlanta, GA 30333, USA , US;(2) Department of Biology, Georgia State University, Atlanta, GA 30333, USA , US |
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Abstract: | Nucleotide sequences of the internal transcribed spacer 2 (ITS2) regions were determined for 13 species within the genus
Candida, representing a collection of those species pathogenic for humans. No two species had identical sequences and the sizes of
ITS2 varied fourfold, representing an apparent continuous gradient of nucleotides. When present, sequence homologies were
observed in the 5′ end of ITS2, and many species exhibited more limited homologies within three known conserved domains found
in other yeasts. Cluster analysis of primary sequence revealed a concordance with a known taxonomic subfamily and suggests
that certain species within the genus form a similar grouping. A majority of species exhibited similar presumptive RNA secondary
structures, consistent with the hypothesis that these spacer regions are essential for correct processing of the 5.8S and
28S subunits.
Received: 14 April 1997 / Accepted: 22 July 1997 |
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