Intestinal ischemia-induced changes in the activity of NADPH-d and energy metabolism enzymes in rat myenteric and splanchnic plexus |
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Authors: | V I Lapsha V N Bocharova L N Utkina V N Gurin |
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Institution: | (1) Institute of Physiology, National Academy of Sciences of Belarus, Minsk, Republic of Belarus |
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Abstract: | The changes in the activity of NADPH-d and energy metabolism enzymes, lactate dehydrogenase (LDG) and succinate dehydrogenase
(SDG), in the neurons of splanchnic and myenteric plexus (SP and MP, respectively), induced by 1-h-long ischemization of a
part of the small intestine, were studied using cytophotometric technique; the measurements were performed under conditions
when synthesis of nitric oxide (NO) was either blocked or activated. The activity of NADPH-d, SDG, and LDG in MP neurons was
shown to be enhanced by ischemia. In SP neurons, the LDG activity increased, while the NADPH-d and SDG activities did not
change. The blockade of NO synthesis with nitro-L-arginine methyl ester was followed by a decrease in the NADPH-d level in
SP and MP neurons, but was maintained at a level lower than the control one during ischemia. Administration of L-arginine,
the NO precursor, increased NADPH-d activity in MP and SP neurons, while at ischemization of the intestine this activity remained
at a level higher than in the control. It is concluded that NO-ergic mechanisms (mostly at the MP level) are significantly
involved in regulation of the functions of the small intestine in ischemia. |
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