An HPLC procedure for monitoring penicillin G fermentations |
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| Authors: | M W Adlard B M Gordon T Keshavarz C Bucke M D Lilly A T Bull G Holt |
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| Institution: | (1) The International Institute of Biotechnology, Biotechnology Division, Faculty of Engineering and Science, Polytechnic of Central London, 115 New Cavendish Street, W1M 8JS London, UK;(2) SERC Centre for Biochemical Engineering, University College London, Torrington Place, WC1E 7JE London, UK;(3) Biological Laboratory, University of Kent, CT2 7NJ Canterbury, Kent, UK |
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| Abstract: | Summary An HPLC procedure for the quantification of the precursor, intermediates, product, and degradation product of penicillin G fermentations is described. The method involves the use of an acetonitrile gradient and direct UV detection with a total run-time of 45 minutes including column regeneration. The technique can be exploited for on-line monitoring of penicillin G fermentations using defined or complex media. |
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