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DNA binding domain of RFX5: Interactions with X-box DNA and RFXANK
Authors:Madhumita Chakraborty  Amitava SenguptaSubrata Banerjee  Abhijit Chakrabarti
Affiliation:Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF, Bidhannagar, Kolkata 700064, India
Abstract:
Keywords:MHC, Major histocompatibility complex   HLA, Human leukocyte antigen   HLA-DRA, Encodes the alpha subunit of MHCII   RFX, Regulatory factor X   RFX5DBD, N-terminal DNA binding domain of RFX5   RFXANK, Regulatory factor X associated ankyrin containing protein   RFXAP, RFX associated protein   UV, Electromagnetic radiation in the ultraviolet region   λmax, Wavelength of maximum fluorescence emission   MALDI, Matrix assisted laser desorption ionization   SDS-PAGE, SDS polyacrylamide gel electrophoresis   SEM, Standard error of the mean   IPTG, Isopropyl β-D-1 thiogalactopyranoside   PMSF, Phenyl methyl sulfonyl fluoride   GST, Glutathione S-transferase   DMS, Dimethyl suberimidate●2 HCl   DTT, Dithiothrietol   CHCA, α-cyano-4-hydroxycinnamic acid   TEA, Triethanolamine   FITC, Fluorescein isothiocyanate   TRITC, Tetramethylrhodamine isothiocyanate   PM, N-(1-pyrene) maleimide   PMF, Peptide mass fingerprint
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