Increase of Lipid Hydroperoxides in Liver Mitochondria and Inhibition of Cytochrome Oxidase by Carbon Tetrachloride -Intoxication in Rats |
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| Authors: | Kazumi Ikeda Miou Toda Kyoko Tanaka Sadako Tokumaru Shosuke Kojo |
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| Affiliation: | a Department of Food Science and Nutrition, Nara Women's University, Nara, Japanb Joetsu University of Education, Joetsu, Niigata, Japan |
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| Abstract: | The cellular localization of lipid hydroperoxides was determined for the first time in mitochondria, microsomes and cytosol of rat liver using a specific method involving chemical derivatization and HPLC. Mitochondria contained the highest level of hydroperoxides. After 6h of intragastric administration of carbon tetrachloride (CCl4) to rats (2 ml/kg body weight), the concentration of lipid hydroperoxides increased significantly in liver mitochondria and cytochrome oxidase activity was inhibited to 35% of the control rats. The mitochondrial content of haem a decreased to 60% of the control at 12 h of CCl4 administration. In vitro reaction of mitochondria with CCl4 caused inactivation of cytochrome oxidase. These observations suggested that cytochrome oxidase and haem a in mitochondria were targets of CCl4. |
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| Keywords: | Carbon tetrachloride hydroperoxide mitochondria lipid peroxidation cytochrome oxidase haem a necrosis |
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