Soybean cyst nematode resistance in soybean is independent of the <Emphasis Type="Italic">Rhg4</Emphasis> locus <Emphasis Type="Italic">LRR-RLK</Emphasis> gene |
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Authors: | Xiaohong Liu Shiming Liu Aziz Jamai Abdelhafid Bendahmane David A Lightfoot Melissa G Mitchum Khalid Meksem |
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Institution: | 1.Division of Plant Sciences and Bond Life Sciences Center,University of Missouri,Columbia,USA;2.Plants and Microbes Genomics and Genetics Lab, Department of Plant Soil and Agricultural Systems,Southern Illinois University at Carbondale,Carbondale,USA;3.Unité de Recherche en Génomique Végétale, UMR INRA-CNRS,Evry Cedex,France;4.Department of Plant Production, College of Food and Agricultural Sciences,King Saud University,Riyadh,Saudi Arabia;5.Center for Excellence, The Illinois Soybean Center,Carbondale,USA |
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Abstract: | To test the function of candidate genes in soybean for resistance to the soybean cyst nematode (SCN), a large collection of
EMS-mutants from the SCN-resistant soybean cultivar “Forrest” was developed for Targeting Induced Local Lesions IN Genomes
(TILLING). Additionally, due to the complexity of the soybean genome, an integrated set of genomic and genetic analysis tools
was employed to complement the TILLING approach. The efficiency of this integrated set of tools was tested using a candidate
soybean gene for resistance to SCN, encoding a leucine-rich repeat receptor-like kinase (LRR-RLK) that was identified by map-based
cloning at the Rhg4 locus. The Rhg4 locus is one of the major quantitative trait loci controlling soybean resistance against SCN race 3 (HG type 0) in cv. Forrest,
but the gene(s) sequence for resistance remains to be determined. Using TILLING, a Forrest mutant containing a nonsense mutation
in the LRR domain of the candidate resistance protein was identified and confirmed; however, the SCN-resistant phenotype of
the mutant was not altered. Haplotyping and EcoTILLING of recombinant inbred lines along with complementation analysis corroborated
the TILLING result and ruled out the possibility of functional redundancy by a second copy of the LRR-RLK gene identified in the soybean genome. This study validates the use of TILLING, in combination with an integrated set of
genomic tools, as an efficient means of testing candidate genes for SCN resistance in soybean. |
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