Enhancing ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae by Mg2+ via reduction in plasma membrane permeability |
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Authors: | Hu Chun-Keng Bai Feng-Wu An Li-Jia |
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Affiliation: | (1) Department of Biological Engineering, Dalian University of Technology, 116024 Dalian, P.R. China;(2) Department of Bioengineering, National Huaqiao University, 362011 Quanzhou, Fujian, P.R. China |
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Abstract: | Mg2+ at 3.5 mM increased the tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae to ethanol. After 9 h of exposure to 20% (v/v) ethanol at 30 °C, all cells died whereas over 50% remained viable for the cells grown with Mg2+. The effect of Mg2+ is closely related to its ability to decrease plasma membrane permeability of cells subjected to ethanol stress. |
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Keywords: | ethanol tolerance magnesium ion membrane permeability coefficient viability |
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