Electroporation of cDNA/Morpholinos to targeted areas of embryonic CNS in <Emphasis Type="Italic">Xenopus</Emphasis> |
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| Authors: | Julien Falk Jovana Drinjakovic Kin Mei Leung Asha Dwivedy Aoife G Regan Michael Piper Christine E Holt |
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| Institution: | (1) Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK;(2) The Queensland Brain InstituteThe University of Queensland, St Lucia, QLD, 4072, Australia |
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| Abstract: | Background Blastomere injection of mRNA or antisense oligonucleotides has proven effective in analyzing early gene function in Xenopus. However, functional analysis of genes involved in neuronal differentiation and axon pathfinding by this method is often
hampered by earlier function of these genes during development. Therefore, fine spatio-temporal control of over-expression
or knock-down approaches is required to specifically address the role of a given gene in these processes. |
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