细菌人工染色体的研究和应用

细菌人工染色体 (Bacterialartificialchromosome ,BAC)是第二代大片段DNA的克隆载体系统。因其嵌合率低 ,遗传稳定性好 ,重组DNA容易分离和制备 ,转化效率高等 ,弥补了YAC的不足 ,很快在基因组研究中处于中心地位。近年来 ,已有多种BAC载体被构建出来 ,这些BAC载体在复杂基因组大片段文库的构建 ,基因的图位克隆 ,基因组物理图谱的构建 ,基因和基因组测序 ,基因组织结构分析 ,染色体组织和进化 ,以及基因的遗传转化和调控研究中得到了广泛的应用。

Progress in Bacterial Artificial Chromosome Research

Bacterial artificial chromosome(BAC) vectors are the secondary system for large DNA fragment cloning.They are over YAC vector with comparatively low levels of chimerism,facility and speed of insert DNA purification,and high genetic stabiity.Therefore,they have been quickly assumed as a central position in genome researches.There are many kinds of BAC vectors be developed recently.The BAC vectors have been contributed to researches of construction of largeinsert genomic libraries,genes map\|based cloning,genomic physical mapping,genomic sequencing,gene and genome organization,chromosome organization and evolution,and gene transformation and regulation.