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Research Note: Simple molecular discrimination of cultivated Porphyra species (Porphyra yezoensis and Porphyra tenera) and related wild species (Bangiales,Rhodophyta)
Authors:Kyosuke Niwa  Atsushi Kobiyama
Institution:1. Fisheries Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, Akashi, Hyogo, 674‐0093, Japan, and;2. Laboratory of Aquatic Microbiology, School of Marine Biosciences, Kitasato University, Sanriku, Ofunato, Iwate, 022‐0101, Japan
Abstract:To discriminate between cultivated Porphyra species (Porphyra yezoensis and Porphyra tenera) and closely related wild Porphyra species, we developed a polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis of the rbcL gene using five restriction enzymes. Although our previous PCR‐RFLP analyses of internal transcribed spacer (ITS) rDNA and plastid RuBisCO spacer regions could not always discriminate wild P. yezoensis, wild P. tenera, and closely related wild species, the PCR‐RFLP profiles of the rbcL gene were useful in discriminating samples collected from natural habitats. Therefore, PCR‐RFLP analysis of the rbcL gene will help in the simple identification of a large number of samples, not only for the establishment of reliable cultures as breeding material, but also for the taxonomic investigations of species that are closely related to cultivated Porphyra.
Keywords:genetic resource  polymerase chain reaction‐restriction fragment length polymorphism  Porphyra yezoensis  Porphyra tenera  rbcL  wild species
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