Blood uridine concentration may be an indicator of the degradation of pyrimidine nucleotides during physical exercise with increasing intensity |
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Authors: | Wioleta Dudzinska Anna Lubkowska Barbara Dolegowska Krzysztof Safranow |
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Institution: | 1.Department of Physiology,Faculty of Natural Sciences of Szczecin University,Szczecin,Poland;2.Department of Biochemistry and Medical Chemistry,Pomeranian Medical University,Szczecin,Poland;3.Department of Laboratory Diagnostics and Molecular Medicine,Pomeranian Medical University,Szczecin,Poland |
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Abstract: | During prolonged maximal exercise, oxygen deficits occur in working muscles. Progressive hypoxia results in the impairment
of the oxidative resynthesis of ATP and increased degradation of purine nucleotides. Moreover, ATP consumption decreases the
conversion of UDP to UTP, to use ATP as a phosphate donor, resulting in an increased concentration of UDP, which enhances
pyrimidine degradation. Because the metabolism of pyrimidine nucleotides is related to the metabolism of purines, in particular
with the cellular concentration of ATP, we decided to investigate the impact of a standardized exercise with increasing intensity
on the concentration of uridine, inosine, hypoxanthine, and uric acid. Twenty-two healthy male subjects volunteered to participate
in this study. Blood concentrations of metabolites were determined at rest, immediately after exercise, and after 30 min of
recovery using high-performance liquid chromatography. We also studied the relationship between the levels of uridine and
indicators of myogenic purine degradation. The results showed that exercise with increasing intensity leads to increased concentrations
of inosine, hypoxanthine, uric acid, and uridine. We found positive correlations between blood uridine levels and indicators
of myogenic purine degradation (hypoxanthine), suggesting that the blood uridine level is related to purine metabolism in
skeletal muscles. |
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