Genetic complementation in hybrid cells derived from two metabolic co-operative defective mammalian cell lines |
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Authors: | C MacDonald |
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Affiliation: | CRC Cell Interactions Group, Departments of Biochemistry, Genetics and Virology, Institute of Genetics, University of Glasgow, Glasgow Gl1 5JS, Scotland |
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Abstract: | A series of hybrid clones have been isolated following the somatic cell fusion of two mammalian cell lines, each defective in junctional transfer of metabolites. One of these parental lines is a variant isolated by selection from the metabolic co-operation competent embryonal carcinoma line PC13TG8. The other parent is LMTK? in which inability to transfer was found to be a pre-existing property. Hybrids between these two cell lines are restored in their ability to co-operate, indicating the existence of at least two genetically distinct lesions affecting metabolic co-operation, each of which is recessive. This is the first demonstration that more than one locus is involved in junctional communication. |
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Keywords: | To whom offprint requests should be addressed. |
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