Evaluation of the relative cell surface charge by using microbial adhesion to hydrocarbon |
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| Authors: | Fatima Hamadi Hassan Latrache Hafida Zahir Jamaa Bengourram Nourreeddine Kouider Abderrahmene Elghmari Khalid Habbari |
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| Institution: | (1) Institute of Food Nutrition and Human Health, Massey University, Palmerston North, New Zealand;(2) Fonterra Research Centre, Palmerston North, New Zealand |
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| Abstract: | A simple and rapid method, Microbial adhesion to hexadecane, for estimating the cell surface charge is proposed. This method
is based on the determination of cell affinity to hexadecane at low ionic strength and at high ionic strength. The difference
between these two affinities can provide the relative cell surface charge. The application of this method for Staphylococcus aureus and Escherichia coli show that the profile of surface charge evolution as a function of pH was similar to these obtained by microelectrophoresis
method. |
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