Effects of human chorionic gonadotropin and N6-O2'-dibutyryl adenosine-3', 5'-monophosphate on phosphofructokinase activity in isolated rat ovaries.

Phosphofructokinase activity in prepubertal rat ovaries is elevated by in vitro treatment with human chorionic gonadotropin or N⁶-O^2′-dibutyryl-adenosine-3′,5′-monophosphate. Puromycin and actinomycin D have no appreciable effect on the hormone-induced enzyme increase but the stimulatory effect of the cyclic nucleotide is blocked by puromycin. In the light of these and other observations, the possible mode of action of the gonadotropin is discussed.