Regulated expression of the tyrosine hydroxylase gene by epidermal growth factor. |
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Authors: | E J Lewis and D M Chikaraishi |
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Institution: | Neuroscience Program, Tufts University School of Medicine, Boston, Massachusetts 02111. |
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Abstract: | The addition of epidermal growth factor (EGF) to cultures of the rat PCG2 pheochromocytoma cell line increased the level of RNA coding for tyrosine hydroxylase (TH). A region of DNA containing 5'-flanking sequences of the TH gene was fused to a heterologous gene and transfected into a rat anterior pituitary cell line, GH4. The TH gene sequences from +27 to -272 contained information sufficient for the induction of TH by EGF. Two regions within this TH DNA were extensively homologous to the EGF regulatory element of the rat prolactin gene. |
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