Measurement of cytokine antibodies. Test development |
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Authors: | Morten Svenson Paul Herbrink |
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Institution: | (1) Institute for Inflammation Research, RHIMA Center, Rigshospitalet, Copenhagen, Denmark;(2) Department of Immunology and Infections, Diagnostic Centre SSDZ, Postbus 5010, 2600 GA Delft, The Netherlands |
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Abstract: | Several assays have been used for detection of antibodies against cytokines. The choice of assay is greatly dependent on the
intended goal, e.g. detection of naturally occurring antibodies or therapy induced antibodies. The different assays can be
grouped in 2 categories. The interference or indirect assays are based on the detection of the test sample interference with
the biological activity, with detection of the cytokine in EIA or with binding to cellular receptors. In direct assays cytokine
antibodies are detected by binding to solid phase fixed cytokines, followed by incubation with a secondary enzyme-labelled
anti-human Ig antibody or by binding to125I-labelled cytokines in RIA. |
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Keywords: | Cytokines antibodies EIA RIA |
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