中国常见仓储书虱PCR-RFLP快速识别

应用PCR-RFLP技术,对我国4种常见仓储书虱即嗜虫书虱Liposcelis entomophila(Enderlein)、嗜卷书虱L.bostrychophila(Badonnel)、无色书虱L.decolor(Pearman)和小眼书虱L.paeta(Pearman)开展快速识别方法的研究。采用CTAB法从上述4种书虱体内提取基因组DNA,选用1对引物扩增16S rDNA基因区域,分别用5种限制性内切酶对PCR产物进行酶切。结果表明,PCR扩增片段大小约为500bp,用限制性内切酶DraI酶切得到的片段可将供试书虱区分开。该方法不受4种供试书虱地理种群、虫态(成虫、若虫)和性别的影响,可用于4种书虱的快速识别。

Rapid discrimination of four common species of store booklice, Liposcelis, from China using PCR-RFLP

Rapid discrimination of four common species of store booklice,Liposcelis entomophila(Enderlein),L.bostrychophila(Badonnel),L.decolor(Pearman)and L.paeta Pearman was conducted by using PCR-RFLP.Genomic DNA was extracted from booklice with CTAB.Genes of 16S rRNA were amplified with specific primers and PCR products were digested with 5 restriction endonucleases respectively.The results showed that the PCR product was estimated to be about 500 bp in length,which could be digested by DraI.All the booklices examined could be discriminated according to the relevant banding patterns.The results were not affected by geographical populations,developmental stages and sexes of the four species.It is suggested that this method is reliable for rapid discrimination of the four booklice species.