Bacteriological activity in unfiltered calf sera collected for tissue culture use |
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| Authors: | Henry C Orr Kyle H Sibinovic Peter G Probst H Donald Hochstein Dewitt C Littlejohn |
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| Institution: | (1) Cellular Physiology Branch, Division of Pathology, Bureau of Biologics, Food and Drug Administration, 8800 Rockville Pike, 20014 Bethesda, Maryland;(2) Present address: Litton Bionetics, Inc., Kensington, Md.;(3) Present address: Division of Control Activities, Bureau of Biologics, FDA, USA |
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| Abstract: | Summary Bacteriological tests were made on 24 lots of unfiltered calf serum collected for subsequent use as a component of tissue
culture media. The examination included the isolation and identification of bacteria, assay of phages, and demonstration of
endotoxin material. Only Gram-positive bacteria were isolated and 96% of the sera were contaminated with bacteria. The prevalent
strains of bacteria found wereBacillus species and streptococci and 63% of the sera coagulatedLimulus amebocyte lysate. More than 90% of the lots contained phages demonstrable with the C-3000 strain ofEscherichia coli. Only one lot of the serum was found to be free from bacteria, phages, and endotoxin by the tests used. |
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