Interaction of Allium sativum leaf agglutinin with midgut brush border membrane vesicles proteins and its stability in Helicoverpa armigera |
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Authors: | Upadhyay Santosh Kumar Mishra Manisha Singh Harpal Ranjan Amol Chandrashekar Krishnappa Verma Praveen Chandra Singh Pradhyumna Kumar Tuli Rakesh |
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Affiliation: | National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, Uttar Pradesh, India. |
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Abstract: | Allium sativum leaf agglutinin (ASAL) binds to several proteins in the midgut of Helicoverpa armigera and causes toxicity. Most of these were glycosylated. Six ASAL-binding proteins were selected for identification. PMF and MS/MS data showed their similarity with midgut aminopeptidase APN2, polycalins and alkaline phosphatase of H. armigera, cadherin-N protein (partial AGAP009726-PA) of Acyrthosiphon pisum, cytochrome P450 (CYP315A1) of Manduca sexta and alkaline phosphatase of Heliothis virescens. Some of the ASAL-binding midgut proteins were similar to the larval receptors responsible for the binding of δ-endotoxin proteins of Bacillus thuringiensis. Galanthus nivalis agglutinin also interacted with most of the ASAL-binding proteins. The ASAL showed resistance to midgut proteases and was detected in the larval hemolymph and excreta. Immunohistochemical staining revealed the presence of ASAL in the body tissue also. |
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Keywords: | Allium sativum leaf agglutinin Animal proteomics Bacillus thuringiensis Glycosylated Helicoverpa armigera Midgut |
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