A general method of deriving the best binding site model consistent with experimental binding data |
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Authors: | J B Pedersen S M Pedersen |
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Affiliation: | Fysik Institut, Odense Universitet, DK-5230 Odense M, and Klinisk Kemisk afd., Odense Sygehus, DK-5000 Odense C, Denmark |
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Abstract: | An analysis of binding data is presented which yields the best binding site model consistent with the experimental data. The analysis is applicable to homotropic binding and yields the number of independent sites, number of interacting sites (dimers and tetramers of sites), intrinsic association constants, and degree of interaction. The information is derived from the roots of a binding polynomial constructed by the fitted Adair constants. |
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Keywords: | Protein - ligand binding Binding data analysis Binding model Homotropic binding Binding - polynomial roots |
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