Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine |
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| Authors: | W C Chang M L Lee C K Chou S C Lee |
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| Institution: | 1. Institute of Biological Chemistry, Academic Sinica, National Taiwan University, Taipei, Taiwan, Republic of China;1. Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan, Republic of China;2. Department of Medical Research, Veterans General Hospital, Taipei, Taiwan, Republic of China |
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| Abstract: | One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72. |
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| Keywords: | polyamide TLC phosphoamino acids |
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