Deletions fusing the i and lac regions of the chromosome in E. coli: isolation and mapping |
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Authors: | Djim Gho and Jeffrey H Miller |
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Institution: | (1) Institut für Genetik der Universität zu Köln, Köln, Germany;(2) Département de Biologie Moléculaire, Université de Genève, Genève, Suisse |
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Abstract: | Summary Three hundred and fifty deletions ending within the i gene (which codes for the lac repressor) at one terminus and within the z gene at the other terminus have been isolated. Twenty-nine of these have been mapped in detail and have endpoints which are distributed throughout the i gene. Two deletions which remove only the very end of the i gene still permit the synthesis of altered repressor molecules retaining some properties of the wildtype lac repressor. In strains carrying these deletions, the synthesis of lac permease is under the control of the i promoter. An additional 40 deletions have been found which apparently fuse the lac permease to an untranslated portion of the terminus of the i-message. |
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