The use of interactive image analysis for demonstrating coexistence

A new approach to establish morphological coexistence using computerized image analysis is described. With this technique, the coexisting pattern in two images is revealed by recording the images via a TV camera on a Zeiss/Kontron IBAS interctive image analyzer. Using an arithmetic or a Boolean algebraic operation, the computer then directly compares the respective patterns obtained for different neuroactive substances and shows the resulting coexisting cells (in white) on a TV-monitor. Also non-coexisting system can be showed in various shades of grey. The method allows for a non-biased, rapid and exact scanning of tissue sections where a possible coexistence may be present.