Preparation of leaf mitochondria from Arabidopsis thaliana |
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Authors: | Olivier Keech ,Pierre Dizengremel, Per Gardeströ m |
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Affiliation: | UmeåPlant Science Centre, Department of Plant Physiology, University of Umeå, UmeåSE-901 87, Sweden; Écologie et écophysiologie forestières, INRA –UHP Nancy-1 (UMR 1137), Équipe écophysiologie cellulaire et moléculaire, BP 239, Vandoeuvre 54 506, France |
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Abstract: | Arabidopsis thaliana is, perhaps, the most important model species in modern plant biology. However, the isolation of organelles from leaves of this plant has been difficult. Here, we present two different protocols for the isolation of mitochondria, yielding either highly functional crude mitochondria or highly purified mitochondria. The crude mitochondria were well coupled with the substrates tested (malate + glutamate, glycine and NADH), exhibiting respiratory control ratios of 2.1–3.9. Purified mitochondria with very low levels of chlorophyll contamination were obtained by Percoll gradient centrifugation, yielding 1.2 mg of mitochondrial protein from 50 g of leaves. |
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