Experiments and theoretical calculations for forming gradients for zonal rotor centrifugation |
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| Authors: | W Leifer T Kreuzer |
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| Affiliation: | Arbeitsgruppe Biochemie, Max-Planck-Institut für experimentelle Medizin, Göttingen, Germany |
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| Abstract: | A gradient mixer operating with a compressed inert gas instead of a pump is designed particularly for work with zonal rotors. Different exponential and linear profiles are calculated as they form along the rotor radius. The centrifugation time dependent on gradient shape and temperature is estimated. |
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