| 牙龈卟啉菌牙龈蛋白酶K的基因克隆和序列分析 |
| |
| 引用本文： | 张凤秋, 杨连甲, 吴织芬, 杨聚才,. 牙龈卟啉菌牙龈蛋白酶K的基因克隆和序列分析[J]. 微生物学通报, 2004, 31(1): 79-81 |
| |
| 作者姓名： | 张凤秋 杨连甲 吴织芬 杨聚才 |
| |
| 作者单位： | 第四军医大学口腔医学院,西安,710032 |
| |
| 摘 要： | 利用PCR方法 ,分别扩增牙龈卟啉菌牙龈蛋白酶K (KGP)的催化结构域 (KGPcd)和凝集素结构域 (KGP-hag)的基因片段 ,将基因片段插入pGEM-T easyVector,通过限制性酶切和核苷酸序列分析鉴定, KGPcd和KGP hag的序列与国外文献报道一致。克隆到牙龈卟啉菌KGP的KGPcd和KGP hag基因 ,为体外表达其活性蛋白奠定了基础。
|
| 关 键 词： | 牙龈卟啉菌 牙龈蛋白酶K 序列测定和分析 |
| 文章编号： | 0253-2654(2004)01-0079-03 |
| 修稿时间： | 2002-11-11 |
CLONING AND SEQUENCING ANALYSIS OF GINGIPAIN K OF PORPHYROMONAS GINGIVALIS |
| |
| ZHANG Feng-Qiu YANG Lian-Jia WU Zhi-Fen YANG Ju-Cai. CLONING AND SEQUENCING ANALYSIS OF GINGIPAIN K OF PORPHYROMONAS GINGIVALIS[J]. Microbiology China, 2004, 31(1): 79-81 |
| |
| Authors: | ZHANG Feng-Qiu YANG Lian-Jia WU Zhi-Fen YANG Ju-Cai |
| |
| Abstract: | The desired DNA product of KGPcd and KGP-hag was obtained from the total DNA of Porphyromonas gingivalis by PCR with two pairs of gene specific primers. The segment of KGPcd and KGP-hag (about 1.5kb and 1.6kb) was inserted into pGEM-T easy Vector. The double-stranded DNA of the postitive clone was analyzed by restriction endonuclease mapping and DNA sequenceing. The sequences of KGPcd and KGP-hag were consistent with those of the references appeared. The proteins of KGPcd and KGP-hag will be obtained for further study. |
| |
| Keywords: | Porphyromonas gingivalis Gingipain K Sequencing and Characterization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
|
点击此处可从《微生物学通报》下载全文 |
