Enhanced sensitivity detection of protein immobilization by fluorescent interference on oxidized silicon |
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Authors: | Volle J-N Chambon G Sayah A Reymond C Fasel N Gijs M A M |
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Institution: | Institute of Microelectronics and Microsystems, Swiss Federal Institute of Technology, CH-1015 Lausanne EPFL, Switzerland. |
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Abstract: | We present a silicon chip-based approach for the enhanced sensitivity detection of surface-immobilized fluorescent molecules. Green fluorescent protein (GFP) is bound to the silicon substrate by a disuccinimidyl terephtalate-aminosilane immobilization procedure. The immobilized organic layers are characterized by surface analysis techniques, like ellipsometry, atomic force microscopy (AFM) and X-ray induced photoelectron spectroscopy. We obtain a 20-fold enhancement of the fluorescent signal, using constructive interference effects in a fused silica dielectric layer, deposited before immobilization onto the silicon. Our method opens perspectives to increase by an order of magnitude the fluorescent response of surface immobilized DNA- or protein-based layers for a variety of biosensor applications. |
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