The Effect of Phosphinothricin (Glufosinate) on Glutathione Synthesis in Plants |
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Authors: | A Herold C Wendler A Wild |
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Abstract: | The inhibitory effect of DL-phosphinothricin (glufosinate) on glutathione synthesis was studied in vivo and in vitro. The influence of phosphinothricin on γ-glutamylcysteine synthetase was compared with the already known effects of l -buthionine sulfoximine and l -methionine sulfoximine. The results showed that phosphinothricin and buthionine sulfoximine are inhibitors of γ-glutamylcysteine synthetase of plants. With both substances the enzyme was inhibited by 50 % at a concentration of 7 . 10?4M (pI50 = 3.15). Methionine sulfoximine reduced the enzyme activity by 50% at 5 . 10?2 M (pI50 = 1.30). It is discussed that the target enzyme of phosphinothricin is the glutamine synthetase whereas the γ-glutamylcysteine synthetase is only an accessory target. |
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Keywords: | Buthionine sulfoximine glufosinate glutathione synthesis methionine sulfoximine phosphinothricin |
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