| Abstract: | In the unicellular green alga Chlorogonium elongatum the level of isocitrate lyase (ICL), the rate of its synthesis and the level of ICL-mRNA measured by in vitro translation are considerably increased after addition of acetate to the culture medium of autotrophically precultured cells. Almost identical increases are obtained independently of whether the cells are cultured after the addition of acetate in the dark (heterotrophically) or in the light (mixotrophically). Transfer of heterotrophic cells to autotrophic conditions results in a fast decrease of ICL-mRNA and ICL protein, while a transfer to mixotrophic conditions causes no alterations in both molecular species. Therefore the concentration of ICL and its translatable mRNA is controlled only by acetate and is unaffected by light. |
