| 普通烟草NtODB基因的电子克隆、表达及生物信息学分析 |
| |
| 引用本文： | 陈涛,彭欣怡,覃剑峰,覃旭,吴密,黄锦媛,黄显雅,危丹妮,王丽萍,金刚. 普通烟草NtODB基因的电子克隆、表达及生物信息学分析[J]. 生物工程学报, 2021, 37(8): 2836-2844 |
| |
| 作者姓名： | 陈涛 彭欣怡 覃剑峰 覃旭 吴密 黄锦媛 黄显雅 危丹妮 王丽萍 金刚 |
| |
| 作者单位： | 广西壮族自治区农业科学院 广西亚热带作物研究所，广西 南宁 530001 |
| |
| 基金项目： | 广西自然科学基金 (No. 2016GXNSFAA380093)，广西农业科学院基本科研业务专项 (Nos. 2020YM57，2021YT153)，广西亚热带作物研究所基本科研业务专项 (No. 桂热研201904) 资助。 |
| |
| 摘 要： | ODB基因在植物同源重组依赖性的DNA双键断裂修复过程中起重要作用,对植物诱变育种具有潜在的应用价值.克隆烟草NtODB基因并分析其表达特征为丰富ODB基因在同源重组DNA修复过程中的作用提供证据.为得到烟草NtODB基因序列,采用电子克隆技术获得该基因cDNA序列并克隆验证.进一步使用生物信息学方法分析该基因表达特征...
|
| 关 键 词： | 普通烟草 NtODB基因 DNA修复 电子克隆 |
| 收稿时间： | 2020-10-19 |
In silico cloning, expression and bioinformatics analysis of NtODB from common tobacco |
| |
| Tao Chen,Xinyi Peng,Jianfeng Qin,Xu Qin,Mi Wu,Jinyuan Huang,Xianya Huang,Danni Wei,Liping Wang,Gang Jin. In silico cloning, expression and bioinformatics analysis of NtODB from common tobacco[J]. Chinese journal of biotechnology, 2021, 37(8): 2836-2844 |
| |
| Authors: | Tao Chen Xinyi Peng Jianfeng Qin Xu Qin Mi Wu Jinyuan Huang Xianya Huang Danni Wei Liping Wang Gang Jin |
| |
| Affiliation: | Guangxi Subtropical Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530001, Guangxi, China |
| |
| Abstract: | It has been reported that ODB genes play an important role in homologous recombination-directed DNA repair, suggesting their potential applications in plant breeding. To analyze the expression characteristics of tobacco NtODB gene, the cDNA sequence of NtODB was obtained using in silico cloning technique. The physicochemical properties, signal peptide, and advanced structures of the predicted protein were analyzed using bioinformatics tools. The results showed that the NtODB gene has a 579-bp open reading frame which encodes a protein with 192 amino acid residues. The protein NtODB is predicted to be alkaline and hydrophilic. Real-time quantitative PCR showed that NtODB was constitutively expressed in different tissues. Subcellular localization showed that NtODB was mainly expressed in cell membrane and chloroplast. These results may help us to better understand and elucidate the roles of ODB genes in the homologous recombination-directed DNA repair. |
| |
| Keywords: | common tobacco NtODB DNA repair in silico cloning |
|
| 点击此处可从《生物工程学报》浏览原始摘要信息 |
|
点击此处可从《生物工程学报》下载全文 |
|
