| 丙型肝炎病毒CS,NS3 NS4区抗原融合基因的构建及其在大肠杆菌中的表达 |
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| 引用本文： | 李铭,沈小川,林矫矫,倪振亚,苑纯秀,刘金明,杨冠珍,蔡幼民,吴祥甫. 丙型肝炎病毒CS,NS3 NS4区抗原融合基因的构建及其在大肠杆菌中的表达[J]. Virologica Sinica, 2001, 0(3) |
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| 作者姓名： | 李铭 沈小川 林矫矫 倪振亚 苑纯秀 刘金明 杨冠珍 蔡幼民 吴祥甫 |
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| 基金项目： | 上海市科技兴农重点攻关项目 (农科攻字 2 0 0 0第 5 8号 ) |
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| 摘 要： | 丙型肝炎病毒是与输血有关的非甲非乙肝病毒[1] ;是全世界输血后获得性肝炎的重要病因之一 ,并与急慢性肝炎、肝硬化和肝癌有关。由于主要通过输血的传播 ,会给人民的身体健康尤其输血事业带来极大危害。采用基因工程技术 ,表达含有多段抗原的融合蛋白作为ＨＣＶ检测试剂的抗原 ,可以简化多种抗原的表达及纯化过程 ,并提高了试剂的均一性[2 ] 。研究表明 ,在ＨＣＶ的抗原基因中 ,核心蛋白Ｃｏｒｅ、ＮＳ3区的Ｃ33ｃ抗原、ＮＳ4区基因编码的抗原免疫原性最强 ,相应抗体出现早 ,分布广 ,亲和力强。因此 ,我们构建了含有中国人ＨＣＶ序列的Ｃｏ…
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| 关 键 词： | 丙型肝炎病毒(HCV) 融合基因 蛋白表达 免疫印迹试验 |
Construction of a Chimeric Gene encoding Core,NS3 and NS4 Region Antigens of Hepatitis C Virus and Its Expression |
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| LI Ming,SHEN Xiao-chuan,LIN Jiao-jiao,NI Zhen-ya,YUAN Chun-xiou,LIU Jin-ming,YANG Guan-zhen,CAI You-min ,WU Xiang-fu. Construction of a Chimeric Gene encoding Core,NS3 and NS4 Region Antigens of Hepatitis C Virus and Its Expression[J]. 中国病毒学(英文版), 2001, 0(3) |
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| Authors: | LI Ming SHEN Xiao-chuan LIN Jiao-jiao NI Zhen-ya YUAN Chun-xiou LIU Jin-ming YANG Guan-zhen CAI You-min WU Xiang-fu |
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| Affiliation: | LI Ming1,SHEN Xiao-chuan2,LIN Jiao-jiao1,NI Zhen-ya1,YUAN Chun-xiou1,LIU Jin-ming1,YANG Guan-zhen2,CAI You-min 1,WU Xiang-fu 2 |
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| Abstract: | DNA fragments of Core(300bp)and NS4(400bp),encoding the nucleocapsid region(Core)protein CS and nonstructural region(NS4)protein NS4a of HCV,have been obtained from HCV genome by PCR,both of the two fragments were liked with C33c(700bp)and formed a chineric gene C33c-Core-NS4(HCV-CCN).The chimeric gene was recombined with expression vector pET24a( )and was expressed in Escherichia coli.The expressed protein was purified by Ni( )NTB affinity chromatography.Its molecular weight was about 58kD.Western blotting analysis showed that the chimerical antigen had good antigenicity,which could play an important role in anti-HCV assay. |
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| Keywords: | Hepatitis C virus(HCV) Fusion gene Protein expression Western blotting analysis |
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