PCR—微孔板杂交法检测不同人群TTV DNA

根据报道的TTV全序列设计引物和探针，建立PCR－微孔板杂交法，检测81例正常人群、92例职业献血员123例甲－庚型肝炎、32例非甲－庚型肝炎、48型发性肝癌患者的TTV DNA。结果表明TTV在以上五种人群中的阳性率分别为3．7％、4．3％、21．1％、28．1％、52．0％，前者与后三者比较有显著性差异（P＜0．05），TTV合并HBV二重感染重叠感染的54．0％，这揭示不同人群均存在TTV感染，正常人群和职业献血员存在健康携带状态，甲－庚型肝炎和非甲－庚肝炎病人为高危人群，TTV可与各型肝炎存在重叠感染，TTV除经血传播外，存在其它传播途径，TTV感染与ALT及TBIL的升高密切相关。

Detection of TTV DNA in Various Populations by Polymerase Chain Reaction-microplate Hybridization

TTV DNA were detected by polymerase chain reaction-microplate bybridization.The results showed that the positive rate of TTV DNA in 81 normal samples,92 blood donation samples,123 A to G hepatitis,32non-A to G hepatitis samples and 48 proimitive hepatic-carcinoma samples was 3.7%?4.3%?21.1%? 28.1% ?52.0%,respectively.There were significant difference between the normal?the hepatitis group and the primitive hepatic-carcinoma group.Among multiinfection,the infection rate of TTV with HBV was as high as 54.0%.The data showed that TTV infection was very popular in different groups.Some of the normal and blood donation group infected with TTV could become virus carrier.And the hepatits patients were high risk group.In addition,TTV may be transmitted by other means besides blood.TTV infection has close relationship with the increase of ALT and TBIL.