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Identification of gibberellin A9 methyl ester as a natural substance regulating formation of reproductive organs in Lygodium japonicum |
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| Authors: | Hisakazu Yamane Nobutaka Takahashi Kiyotoshi Takeno Masaki Furuya |
| Affiliation: | (1) Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, 113 Bunkyo-ku, Tokyo, Japan;(2) Botany Department, Faculty of Science, University of Tokyo, 113 Bunkyo-ku, Tokyo, Japan;(3) Present address: Department of Biology, The University of Calgary, T2N 1N4 Calgary, Alberta, Canada |
| Abstract: | Prothallia of Lygodium japonicum (Thunb.) Sw. were aseptically cultured under white light in a mineral solution. Solvent fractionation of the resultant culture medium and subsequent preparative thinlayer chromatography yielded a fraction that induced antheridium formation and inhibited archegonium formation. Combined gas chromatography-selected ion monitoring analysis of this fraction confirmed the presence of gibberellin A9 methyl ester (GA9-me) as an antheridiogen and an inhibitor of archegonium formation. Exogenously applied [3H]GA9 was rapidly converted to [3H]GA9-me in the prothallial tissue. Authentic GA9-me was active to 10-10M in antheridium formation and to 10-9M in the inhibition of archegonium formation.Abbreviations GAs gibberellins - GAn gibberellin An - GAn me, gibberellin An methyl ester - TLC thin-layer chromatography - GCSIM Combined gas chromatography-selected ion monitoring |
| Keywords: | Antheridiogen Archegonium formation Gibberellin Lygodium |
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