The nucleotide sequence of a cDNA clone containing the entire coding region for mouse X-chromosome-linked phosphoglycerate kinase |
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| Authors: | N Mori J Singer-Sam C Y Lee A D Riggs |
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| Affiliation: | 1. Department of Molecular Biology and Reproductive Genetics, The Beckman Research Institute of the City of Hope, Duarte, CA 91010 U.S.A. Tel. (818) 359-8111 ext. 2422;2. Department of Molecular Biology Andrology Laboratory, Acute Care Unit, University of British Columbia, Vancouver, BCCanada V6T2B5 Tel. (604) 228-7023;1. Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK;2. Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK;3. Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, UK;1. Department of Molecular Biology, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077 Göttingen, Germany;2. Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK;3. Bioanalytical Mass Spectrometry, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077 Göttingen, Germany;4. Institute of Clinical Chemistry, University Medical Center Göttingen, 37075 Göttingen, Germany;1. Center for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland;2. Department of Physiology, University of Lausanne, 1005 Lausanne, Switzerland;3. Institute of Sport Sciences, University of Lausanne, 1015 Lausanne, Switzerland;4. IGMM, Université de Montpellier, UMR 5535 CNRS, 34293 Montpellier, France;5. Nestlé Institute of Health Sciences SA, EPFL Innovation Park, 1015 Lausanne, Switzerland;6. École Polytechnique Fédérale de Lausanne, School of Life Sciences, 1015 Lausanne, Switzerland;7. Ludwig Center for Cancer Research, University of Lausanne, 1066 Epalinges, Switzerland;8. Institut Cochin, INSERM U1016, Paris, France;9. CNRS, UMR 8104, Paris, France;10. Université Paris Descartes, Sorbonne Paris Cité, Paris, France;11. School of Life Sciences, University of Dundee, Dundee, Scotland, UK |
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| Abstract: | A clone containing cDNA for X chromosome-linked phosphoglycerate kinase (PGK-1) was isolated from a mouse myeloma cDNA library. The nucleotide (nt) sequence of the cDNA has been determined, and the amino acid (aa) sequence of the enzyme thereby deduced. At the nt level, the coding region of mouse PGK cDNA has 93% homology with human X-linked cDNA and 60% homology with the yeast gene. Mouse PGK-1 protein contains 416 aa and is 98%, 96% and 64% homologous with human, horse, and yeast enzyme sequences, respectively. |
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