Susceptibility of infant mice to F5 (K99)E. coli infection: Differences in glycosyltransferase activities in intestinal mucosa of inbred CBA and DBA/2 strains |
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Authors: | Philippe A Grange Michèle Mouricout |
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Institution: | (1) Laboratoire de Biochimie, UER des Sciences, 123 Avenue A. Thomas, 87060 Limoges, Cedex, France |
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Abstract: | EnterotoxigenicEscherichia coli (ETEC) strains expressing F5 (K99) fimbriae cause diarrhoea in the young animal through adhesion to specific sialoglycolipids of the small intestine surface. We studied here an infant mouse diarrhoea model, as CBA infant mice are susceptible to F5-positive ETEC infection, whereas DBA/2 ones are resistant. In an attempt to determine an enzymatic basis for susceptibility and resistance, we investigated the intestine ganglioside pattern in relation to the activity of glycosyltransferases responsible for the globo- and ganglio-series. We observed that the intestine of susceptible CBA infant mice displayed a characteristic sialoglycolipid pattern containing mainly the F5 receptors. The two murine strains differed in the relative activities of galactosyltransferases (GbOse3Cer and GM1 synthases),N-acetylgalactosylaminyltransferases (GA2 and GM2 synthases) and sialyltransferases (GM3 and GD3 synthases). An elevated GM3-synthase activity was observed in the intestine of susceptible CBA infant mice, at the age of high susceptibility. Hence, we conclude that the marked specificity of mouse type correlated with susceptibility and resistance to F5-positive ETEC infection which could be controlled through the regulation of glycosyltransferase activities.Abbreviations NeuAc
N-acetylneuraminic acid
- NeuGc
N-glycolylneuraminic acid
- Glc
glucose
- GalNAc
N-acetylgalactosamine
- Gal
galactose
- Car
ceramide
- LacCer
lactosylceramide (Galß-4Glcß1-1Cer)
- GA2
asialo-GM2 (GgOse3Cer)
- GA1
asialo-GM1 (GgOse4Cer)
- NeuAc/NeuGc-GMla
II3 NeuAc/NeuGc-GgOse4Cer
- NeuAc/NeuGc-GM1a
IV3 NeuAc/NeuGc-GgOse4Cer
- NeuAc/NeuGc-GM2
II3 NeuAc/neuGc-GgOse3Cer
- NeuAc/NeuGc-GM3, II3 NeuAc/NeuGc-LacCer; NeuAc/NeuGc-GD1a, IV3 NeuAc/NeuGc, II3 NeuAc/NeuGc-GgOse4Cer; NeuAc/NeuGc-GD1b
II3 (NeuAc/NeuGc)2-GgOse4Cer
- NeuAc/NeuGc-GD1c
IV3 (NeuAc/NeuGc)2-GgOse4Cer
- NeuAc/NeuGc-GD2, II3 (NeuAc/NeuGc)2-GgOse3Cer; NeuAc/NeuGc-GD3, II3 (NeuAc/NeuGc)2-Lac Cer; NeuAc/NeuGcGT1a
IV3 (NeuAc/NeuGc)2, II3 NeuAc/NeuGc-GgOse4Cer
- NeuAc/neuGc-GT1b
IV3 NeuAc/NeuGc, II3 (NeuAc/NeuGc)2-GgOse4Cer
- NeuAc/NeuGc-GT1c
II3 (NeuAc/NeuGc)3-GgOse4Cer; NeuAc/NeuGc-GT2, II3 (NeuAc/NeuGc)3-GgOse3Cer
- NeuAc/NeuGc-GT3
II3 (NeuAc/NeuGc)3-Lac Cer
- NeuAc/NeuGc-GQ1b
IV3 (NeuAc/NeuGc)2, II3 (NeuAc/NeuGc)2-GgOse4Cer
- NeuAc/NeuGc-GQ1c
IV3 NeuAc/NeuGc, II3 (NeuAc/NeuGc)3-GgOse4Cer
- NeuAc/NeuGc-GP1c
IV3 (NeuAc/NeuGc)2, II3 (NeuAc/NeuGc)3-GgOse4Cer
- GD, GT and GQ
di-, tri- and tetra-sialoglangliosides. NeuGc-SPG, IV3 NeuGc-nLcOse4Cer. Glycosyltransferases assayed in this work areN-acetylgalactosaminyltransferases
- UDP-GalNAc
lactosylceramide 1-4N-acetylgalactosaminyltransferase or GA2 synthase (EC 2.4.1-) and UDP-GalNAc:(N-acetylneuraminyl)-lactosylceramide 1-4N-acetylgalactosaminyltransferase or GM2 synthase (EC 2.4.1.92)
- sialyltransferases
CMP-N-acetylneuraminate: lactosylceramide 2–3 sialyltransferase (sialyltransferases I and IV) or GM3 synthase (EC 2.4.99.-) and CMP-N-acetylneuraminate:(N-acetylneuraminyl) lactosylceramide 2-8 sialyltransferase (sialyltransferase II) or GD3 synthase (EC 24.99.8)
- galactosyltransferases
UDP-galactose:N-acetylgalactosaminyl-(N-acetylneuraminyl) lactosylceramide 1-3 galactosyltransferase (galactosyltransferase II) or GM1a synthase (EC 2.4.1.62) and UDP-galactose:lactosylceramide 1-4 galactosyltransferase or GbOse3Cer synthase (EC 2.4.1-) |
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Keywords: | EnterotoxigenicE coli F5 positive-ETEC inbred mice glycosyltransferases glycolipids intestine |
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