Automated determination of rifampicin in plasma samples by in-tube solid-phase microextraction coupled with liquid chromatography |
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Authors: | Melo L P Queiroz R H C Queiroz M E C |
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Affiliation: | Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeir?o Preto, Universidade de S?o Paulo, Ribeir?o Preto 14040-901, SP, Brazil. |
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Abstract: | A sensitive and automated method is described for determination of rifampicin in plasma samples for therapeutic drug monitoring by in-tube solid-phase microextraction coupled with liquid chromatography (in-tube SPME/LC). Important factors in the optimization of in-tube SPME are discussed, such as coating type, sample pH, sample draw/eject volume, number of draw/eject cycles, and draw/eject flow rate. Analyte pre-concentrated in the polyethylene glycol phase was directly transferred to the liquid chromatographic column by percolation of the mobile phase, without carryover. The method was linear over the 0.1-100 μg/mL range, with a linear coefficient value (r(2)) of 0.998. The inter-assay precision presented coefficient of variation ≤ 1.7%. The effectiveness and practicability of the proposed method are proven by analysis of plasma samples from ageing patients undergoing therapy with rifampicin. |
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