The cellulase of Trichoderma viride: Separation of the components involved in the solubilization of cotton |
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Authors: | K. Selby and C. C. Maitland |
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Affiliation: | Shirley Institute, Didsbury, Manchester 20 |
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Abstract: | 1. Culture filtrates from Trichoderma viride have been fractionated by gel filtration on Sephadex G-75 followed by ion-exchange chromatography on DEAE- and SE-Sephadex. 2. The components essential for attack on cotton are a carboxymethylcellulase, a cellobiase and a third (C1) component which has no action on CM-cellulose, cellobiose or cotton. 3. These components, which together can completely convert cotton into water-soluble products, lose this ability when separated and regain it quantitatively when recombined in their original proportions. |
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