| 新型戊肝病毒ORF3和部分ORF1基因的序列分析 |
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| 引用本文： | 王佑春,张华远,辜文杰,Roger Ling,李河民,Tim J Harrison. 新型戊肝病毒ORF3和部分ORF1基因的序列分析[J]. 中国病毒学, 2001, 16(1): 28-33 |
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| 作者姓名： | 王佑春 张华远 辜文杰 Roger Ling 李河民 Tim J Harrison |
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| 作者单位： | 1. 中国药品生物制品检定所,
2. Center for Hepatology, Royal Free Campus,University College London, Hampstead , London NW3 2PF,UK |
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| 基金项目： | 部分由英国WellcomeTrust基金组织资助 |
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| 摘 要： | 用RT-PCR方法对2例感染新型HEV的样品进行了检测，并对PCR产物进行了克隆及测序。检测结果显示用常规PCR检测易造成ORF3中GC丰富区的缺失，但在常规PCR反应液中加入GCmelt溶液可成功地扩增GC丰富区。序列分析显示T1和T11属于同一基因型，但不同于已报道的HEVI型、II型和III型，为一新的基因型。T1和T11与I型在该区的核苷酸同源性为79%～82%；与II型的同源性为80%～81%;和III型的同源性为83%～85%。
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| 关 键 词： | 戊型肝炎病毒；基因型； 同源性；开放读码框架 |
| 文章编号： | 1003-5125(2001)01-0028-06 |
| 修稿时间： | 1999-12-06 |
Sequence Analysis of ORF3and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis EVirus(HEV) |
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| WANG You-chun,ZHANG Hua-yuan,GU Wen-jie,Roger Ling,Li He-min,Tim J Harrison. Sequence Analysis of ORF3and Partial ORF1 Region from Two Patients Infected with New Genotype of Hepatitis EVirus(HEV)[J]. Virologica Sinica, 2001, 16(1): 28-33 |
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| Authors: | WANG You-chun ZHANG Hua-yuan GU Wen-jie Roger Ling Li He-min Tim J Harrison |
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| Abstract: | ORF3 and partial ORF1 regions were amplified with RT-PCR f rom two patients (T1 and T11)infected with new genotype of hepatitis E Virus. Th e PCR products were cloned and sequenced. The results showed that G-C rich regi on in ORF3 was deleted when amplified with normal PCR reaction. However, PCR rea ction containing G-C melt solution can overcome this problem. The sequence anal ysis showed that T1 and T11 belong to a new genotype of HEV which differs from g enotype I,II and III reported.T1 and T11 have 79%～82%, 80%～81% and 83%～85% id entical to genotype I,II and III respectively. |
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